prof. RNDr. Břetislav Brzobohatý, CSc.

Curriculum vitae

Person Identification

• RNDr. Břetislav Brzobohatý, CSc.
  born September 27, 1957 in Brno, married, 2 children

Workplace

• Laboratory of Plant Molecular Physiology
  Masaryk University
  Faculty of Science
  Kotlářská 2
  611 37 Brno, Czech Republic

Employment Position

• Head of the Laboratory of Plant Molecular Physiology

Education and Academic Qualifications

• 1986: CSc., biochemistry, Institute of Animal Physiology Slovak Academy of Sciences, Ivánka pri Dunaji, and University of J.E. Purkyně, Brno, Czechoslovakia, Prof. Ladislav Kováč, advisor
• 1981: RNDr, biochemistry, University of J.E. Purkyně, Brno, Czechoslovakia, Dr. Jan Kovář, advisor

Employment Summary

• 8/96 - present: head of Laboratory of Plant Molecular Physiology, Faculty of Science, Masaryk University, Brno, Czech Republic
• 7/93 - present: group leader, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
• 7/85-6/89: scientific worker, Institute of Biophysics, Czechoslovak Academy of Sciences, Brno, Czechoslovakia

Teaching Activities

• since 1995: lecturer at the Department of Biochemistry and the Department of Genetics and Molecular Biology, Faculty of Science, Masaryk University, Brno, Czech Republic

Projects

• 522/96/K116: Grant Agency of the Czech Republic, Brzobohatý, B. (principal investigator): Development of regulatable pollen sterility.
• 206/96/K188: Grant Agency of the Czech Republic, Macháčková, I. (principal investigator): Cytokinins and auxins in regulation of plant development. (Collaborator: B.Brzobohatý).
• VS 96096: Ministry of Education of the Czech Republic, Brzobohatý, B. (principal investigator): Laboratory of Plant Molecular Physiology.
• A5004603: Grant Agency of the Academy of Sciences of the Czech Republic, Brzobohatý, B. (principal investigator): Structure-function relationship in maize beta-glucosidase specific for cytokinin glucosides: the role of cysteine residues.
• PL966135: European Commission, DG XII, programme INCO-COPERNICUS, Palme, K. (principal investigator): The Role of Phytohormones in Control of Plant Development and Architecture.(Collaborator: B. Brzobohatý).
• PL966135: National Science Foundation, USA, Raikhel, N.V. (US principal investigator), Brzobohatý, B. (Czech principal investigator): U.S. - Czech Plant Research on Subcellular Compartmentation of Phytohormone Conjugation.
• RS PDF/BLL: Royal Society, UK Moore, I. (UK principal investigator), Brzobohatý, B. (Czech principal investigator): The role of cytokinin metabolism in plant growth and development.

Academical Stays

• 12/87-4/88: Developmental Molecular Biology, study stay in Dr. J.-A. Lepesant´s laboratory, Institut Jacques Monod, Universite Paris VII, France
• 7/89-12/94: Plant Molecular Biology, postdoctoral stay at the Max-Planck-Institute for Plant Breeding, Cologne, Germany, Prof. J. Schell, Dr. K. Palme
• 4-7/97, 1-3/98 and 5-8/98: visiting scientist at the Department of Plant Sciences, University of Oxford, UK, collaborative research project with Dr. Ian Moore, "The role of cytokinin metabolism in plant growth and development", supported by the Royal Society
• 8-12/97 and 9-12/98: visiting scientist at the MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, USA, collaborative research project with Prof. N.V. Raikhel, "Subbcellular Compartmentation of Phytohormone Conjugation", supported by the National Science Foundation

University Activities

• 1996 - present: head of the Laboratory of Plant Molecular Physiology

Other Activities

• 7/93 - present: group leader, Institute of Biophysics, Academy of Sciences of the Czech Republic, Brno, Czech Republic
• 7/85-6/89: scientific worker, Institute of Biophysics, Czechoslovak Academy of Sciences, Brno, Czechoslovakia

Appreciation of Science Community

• member of the Federation of European Societies of Plant Physiology
• member of the Czech Committee for Biochemistry and Molecular Biology
• member of the Czech Society for Biochemistry and Molecular Biology

Selected Publications

• VÉVODOVÁ, Jitka, Jaromír MAREK, Jan ZOUHAR, Břetislav BRZOBOHATÝ a X.-D. SU. Purification, Crystallization and Preliminary X-ray Analysis of a Maize Cytokinin-glucoside-specific b-Glucosidase. Acta Crystallogr., Sect.D. Dánsko, 2001, D57, č. 1, s. 140-142. ISSN 0907-4449. info
• LEXA, Matej, Jakub HORÁK a Břetislav BRZOBOHATÝ. Virtual PCR. Bioinformatics. Oxford: Oxford University Press, 2001, roč. 17, č. 2, s. 192-193. ISSN 1367-4803. VPCR Homepage info
• ZOUHAR, Jan, Jitka VÉVODOVÁ, Jaromír MAREK, Jiří DAMBORSKÝ, X.-D. SU a Břetislav BRZOBOHATÝ. Insights into the Functional Architecture of the Catalytic Center of a Maize Beta-Glucosidase Zm-p60.1. Plant Physiology. USA: American Society of Plant Physiologists, 2001, roč. 127, č. 3, s. 973-985. ISSN 0032-0889. info
• KRISTOFFERSEN, Peter, Břetislav BRZOBOHATÝ, Ingo HÖHFELD, Laszlo BAKO, Michael MELKONIAN a Klaus PALME. Developmental regulation of the maize Zm-p60.1 gene encoding a beta-glucosidase located to plastids. Planta. Springer Berlin Heidelberg 2000, 2000, roč. 210, č. 3, s. 407-415. ISSN 0032-0935. info
• KUDEROVÁ, Alena, Elizabeth NANAK, Martin TRUKSA a Břetislav BRZOBOHATÝ. Use of Rifampicin in T7 RNA Polymerase-Driven Expression of a Plant Enzyme. Rifampicin Improves Yield and Assembly. Protein Expression and Purification. San Diego, California: Academic Press, 1999, roč. 16, č. 3, s. 405-409. ISSN 1046-5928. info
• BENKOVÁ, Eva, Erwin WITTERS, Walter VAN DONGEN, Jan KOLAR, Václav MOTYKA, Břetislav BRZOBOHATÝ, Harry A. VAN ONCKELEN a Ivana MACHÁČKOVÁ. Cytokinins in Tobacco and Wheat Chloroplasts. Occurence and Changes Due to Light/Dark Treatment. Plant Physiology. USA: American Society of Plant Physiologists, 1999, roč. 121, s. 245-251. ISSN 0032-0889. info
• ZOUHAR, Jan, Elizabeth NANAK a Břetislav BRZOBOHATÝ. Expression, Single-Step Purification and Matrix-Assisted Refolding of a Maize Cytokinin Glucoside-Specific beta-Glucosidase. Protein Expression and Purification. San Diego, California: Academic Press, 1999, roč. 1999, č. 17, s. 153-162. ISSN 1046-5928. info
• ROTREKL, Vladimír, Eliška NEJEDLÁ, Igor KUČERA, Fuad ABDALLAH, Klaus PALME a Břetislav BRZOBOHATÝ. The role of cysteine residues in structure and enzyme activity of a maize beta-glucosidase. European Journal of Biochemistry. 1999, roč. 266, č. 3, s. 1056-1065. ISSN 0014-2956. info
• DUBOVÁ, Jaroslava, Petr MLEJNEK a Břetislav BRZOBOHATÝ. Dynamics of b-glucosidase Zm-p60.1 ectopic expression during transgenic pollen development: a histochemical approach. General Physiology and Biophysics. Bratislava: Slovak Academy of Sciences, 1999, roč. 18, Suppl.1, s. 112-116. ISSN 0231-5882. info
• SUTTER-CRAZZOLARA, C., B. BRZOBOHATÝ, B. GAZDOVÁ, J. SCHELL a B. REISS. T-DNA integrations in a new family of repetitive elements of Nicotiana tabaccum. Journal of Molecular Evolution. 1995, roč. 41, s. 498-504. info
• BRZOBOHATÝ, B., I. MOORE a K. PALME. Cytokinin metabolism: implications for regulation of plant growth and development. Plant Molecular Biology. 1994, roč. 26, s. 1483-1497. ISSN 0167-4412. info
• BRZOBOHATÝ, B., I. MOORE, P. KRISTOFFERSEN, L. BAKO, N. CHAMPOS, J. SCHELL a K. PALME. Release of activite cytokinin by a beta-glucosidase localized to the maize root meristem. Science. 1993, roč. 262, s. 1051-1054. ISSN 0036-8075. info
• HESSE, T., C. GARBERS, B. BRZOBOHATÝ, G. KREIMER, D. SÖLL, M. MELKONIAN, J. SCHELL a K. PALME. Two members of the ERabp gene family are expressed differentially in reproductive organs but to similar levels in coleoptile of maize. Plant Molecular Biology. 1993, roč. 23, s. 57-66. ISSN 0167-4412. info
• MOORE, I., J. FELDWISH, N. CAMPOS, R. ZETTL, B. BRZOBOHATÝ, L. BAKO, J. SCHELL a K. PALME. Auxin-binding proteins of Zea mays identified by photoaffinity labelling. Biochemical Society Transaction. 1992, roč. 20, č. 1, s. 70-73. info
• BEZDĚK, M., B. KOUKALOVÁ, B. BRZOBOHATÝ a B. VYSKOT. 5-Azacytidine induced hypomethylation of tobacco HRS60 tandem DNA repeats in tissue culture. Planta. Springer Berlin Heidelberg 2000, 1991, roč. 184, s. 487-490. ISSN 0032-0935. info
• BRZOBOHATÝ, B. a L. KOVÁČ. Genetic transformation of yeast protoplasts with DNA encapsulated in liposomes. FEMS Microbiology Leetters. 1986, roč. 35, s. 29-31. info
• BRZOBOHATÝ, B. a L. KOVÁČ. Interaction of plasmid DNA with yeast protoplasts and a mechanism of genetic transformation. FEBS Lett. 1985, roč. 183, s. 211-214. info

1999/06/17